Research Highlight Keys to Cultivating Uncultured Microbes: Elaborate Enrichment Strategies and Resuscitation of Dormant Cells
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Keys to Cultivating Uncultured Microbes: Elaborate Enrichment Strategies and Resuscitation of Dormant Cells
Over the last few years, extensive investigations have been performed in an attempt to isolate yet-to-be cultivated microorganisms that are of ecological importance (1, 4, 12, 19). The enrichment, isolation, characterization, and genome sequencing of these organisms are classical, but robust processes that explicitly unveil their entity (5, 6, 9). However, difficulties are associated with the d...
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Under gradual acidification of growth medium resulting in the formation of dormant Mycobacterium smegmatis, a significant accumulation of free trehalose in dormant cells was observed. According to 1H- and 13C-NMR spectroscopy up to 64% of total organic substances in the dormant cell extract was represented by trehalose whilst the trehalose content in an extract of active cells taken from early ...
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